Inflammatory markers and incident frailty in men and women:the English Longitudinal Study of Ageing

Cross-sectional studies show that higher blood concentrations of inflammatory markers tend to be more common in frail older people, but longitudinal evidence that these inflammatory markers are risk factors for frailty is sparse and inconsistent. We investigated the prospective relation between baseline concentrations of the inflammatory markers C-reactive protein (CRP) and fibrinogen and risk of incident frailty in 2,146 men and women aged 60 to over 90 years from the English Longitudinal Study of Ageing. The relationship between CRP and fibrinogen and risk of incident frailty differed significantly by sex (p for interaction terms <0.05). In age-adjusted logistic regression analyses, for a standard deviation (SD) increase in CRP or fibrinogen, odds ratios (95 % confidence intervals) for incident frailty in women were 1.69 (1.32, 2.17) and 1.39 (1.12, 1.72), respectively. Further adjustment for other potential confounding factors attenuated both these estimates. For an SD increase in CRP and fibrinogen, the fully-adjusted odds ratio (95 % confidence interval) for incident frailty in women was 1.27 (0.96, 1.69) and 1.31 (1.04, 1.67), respectively. Having a high concentration of both inflammatory markers was more strongly predictive of incident frailty than having a high concentration of either marker alone. In men, there were no significant associations between any of the inflammatory markers and risk of incident frailty. High concentrations of the inflammatory markers CRP and fibrinogen are more strongly predictive of incident frailty in women than in men. Further research is needed to understand the mechanisms underlying this sex difference

The Cost in Fatalities, Injuries and Crashes Associated with Waiting to Deploy Vehicle-to-Vehicle Communication

Today, the auto industry is ready to deploy vehicle-to-vehicle (V2V) communication-based safety systems employing proven technology and well-developed standards. In fact, the readiness entails more than just V2V, but potentially vehicle-to-everything (V2X) communication, where everything (X) can include communication with other vehicles, the roadway infrastructure, and vulnerable road users.The purpose of this white paper is not to assess the strength or weaknesses of either DSRC or C-V2X technology. Rather it is to investigate and quantify the lost opportunities to prevent light-vehicle crashes by waiting for the development and implementation of CV2X as opposed to mandating DSRC now in the form of FMVSS 150. The focus on V2V crashes, as opposed to all crashes, is based on significant levels of uncertainty associated with the deployment of infrastructure-based hardware and/or small-cell networks that would be required for either DSRC or C-V2X technologies to address many of the single vehicle crash types.https://deepblue.lib.umich.edu/bitstream/2027.42/147434/1/The Cost Associated with Waiting to Deploy DSRC 032018.pd

Some implications of sampling choices on comparisons between satellite and model aerosol optical depth fields

The comparison of satellite and model aerosol optical depth (AOD) fields provides useful information on the strengths and weaknesses of both. However, the sampling of satellite and models is very different and some subjective decisions about data selection and aggregation must be made in order to perform such comparisons. This work examines some implications of these decisions, using GlobAerosol AOD retrievals at 550 nm from Advanced Along-Track Scanning Radiometer (AATSR) measurements, and aerosol fields from the GEOS-Chem chemistry transport model. It is recommended to sample the model only where the satellite flies over on a particular day; neglecting this can cause regional differences in model AOD of up to 0.1 on monthly and annual timescales. The comparison is observed to depend strongly upon thresholds for sparsity of satellite retrievals in the model grid cells. Requiring at least 25% coverage of the model grid cell by satellite data decreases the observed difference between the two by approximately half over land. The impact over ocean is smaller. In both model and satellite datasets, there is an anticorrelation between the proportion <i>p</i> of a model grid cell covered by satellite retrievals and the AOD. This is attributed to small <i>p</i> typically occuring due to high cloud cover and lower AODs being found in large clear-sky regions. Daily median AATSR AODs were found to be closer to GEOS-Chem AODs than daily means (with the root mean squared difference being approximately 0.05 smaller). This is due to the decreased sensitivity of medians to outliers such as cloud-contaminated retrievals, or aerosol point sources not included in the model

Assessment of a Driver Interface for Lateral Drift and Curve Speed Warning Systems: Mixed Results for Auditory and Haptic Warnings

Lateral Drift Warning (LDW) and Curve Speed Warning (CSW)systems were developed to address two main critical events in run-off-roadcrashes, which are road edge departure and excessive speed. The LDW systemused a two-stage alert system, with the first stage activating when the driverdeparted a lane with a dashed boundary and the imminent, or second stage, whendeparting a lane with a solid boundary. The CSW also employed a two-stage alert,with the level based on the degree of over-speed for the upcoming curve. Thehaptic modality, in the form of seat vibration, was chosen as the first levelwarning for both systems, and auditory was chosen as the second or most urgentlevel. The two systems were installed in a fleet of instrumented vehicles andloaned to 78 randomly selected licensed drivers for approximately 4 weeks.Debriefing questions detailing the driver’s experience with the system wereadministered and analyzed in a two by two design of modality by system. Afterexamination of both the statistical results and the open-ended comments, thequestion of which modality is most appropriate is still uncertain. Each modalityhad positive aspects. Haptic does not alert the entire car and participants alsoconsidered it less distracting. Auditory provided better recognition betweenwarnings and participants were better able to understand the meaning and therequired response for each warning

Trust-Fund Doctrine Revisited [Part I]

The foundation case in Amenca was an action to restore dividends paid out to shareholders by an insolvent bank, but from limited beginnings the trust-fund doctrine expanded to become a major legal weapon for corporate creditors under a wide variety of conditions. In this discussion we propose to treat separately three major aspects of the trust-fund problem: (1) the status of corporate insolvency, which was a condition precedent to the operation of the rule; (2) the rights of creditors against creditors; and (3) the rights of creditors against shareholders. Our analysis will be confined chiefly to the statutory and case law of the state of Washington with no attempt at detailed treatment of the rules of other courts beyond an incidental comparison. The Federal Bankruptcy Act is excluded from analytical discussion as deserving separate treatment. We undertake no more in that regard than to point out to the practitioner the possibility of using substantive state law to advantage in proceedings under the Federal Act. The body of case authority will be appraised in the light of the various state statutes which apply, and an attempt made to indicate the current place of the trust-fund doctrine in this jurisdiction

Effects of higher dietary protein and fiber intakes at breakfast on postprandial glucose, insulin, and 24-H interstitial glucose in overweight adults

Dietary protein and fiber independently influence insulin-mediated glucose control. However, potential additive effects are not well-known. Men and women (n = 20; age: 26 ± 5 years; body mass index: 26.1 ± 0.2 kg/m²; mean ± standard deviation) consumed normal protein and fiber (NPNF; NP = 12.5 g, NF = 2 g), normal protein and high fiber (NPHF; NP = 12.5 g, HF = 8 g), high protein and normal fiber (HPNF; HP = 25 g, NF = 2 g), or high protein and fiber (HPHF; HP = 25 g, HF = 8 g) breakfast treatments during four 2-week interventions in a randomized crossover fashion. On the last day of each intervention, meal tolerance tests were completed to assess postprandial (every 60 min for 240 min) serum glucose and insulin concentrations. Continuous glucose monitoring was used to measure 24-h interstitial glucose during five days of the second week of each intervention. Repeated-measures ANOVA was applied for data analyses. The HPHF treatment did not affect postprandial glucose and insulin responses or 24-h glucose total area under the curve (AUC). Higher fiber intake reduced 240-min insulin AUC. Doubling the amount of protein from 12.5 g to 25 g/meal and quadrupling fiber from 2 to 8 g/meal at breakfast was not an effective strategy for modulating insulin-mediated glucose responses in these young, overweight adults.T32 HL116276 - NHLBI NIH HHS; UL1 TR001108 - NCATS NIH HH

Evaluation of NAD(+)-dependent DNA ligase of mycobacteria as a potential target for antibiotics

Mycobacteria contain genes for several DNA ligases, including ligA, which encodes a NAD+-dependent enzyme that has been postulated to be a target for novel antibacterial compounds. Using a homologous recombination system, direct evidence is presented that wild-type ligA cannot be deleted from the chromosome of Mycobacterium smegmatis. Deletions of native ligA in M. smegmatis could be obtained only after the integration of an extra copy of M. smegmatis or Mycobacterium tuberculosis ligA into the attB site of the chromosome, with expression controlled by chemically inducible promoters. The four ATP-dependent DNA ligases encoded by the M. smegmatis chromosome were unable to replace the function of LigA. Interestingly, the LigA protein from M. smegmatis could be substituted with the NAD+-dependent DNA ligase of Escherichia coli or the ATP-dependent ligase of bacteriophage T4. The conditional mutant strains allowed the analysis of the effect of LigA depletion on the growth of M. smegmatis. The protein level of the conditional mutants was estimated by Western blot analysis using antibodies raised against LigA of M. tuberculosis. This revealed that a strong overproduction or depletion of LigA did not affect the growth or survival of mycobacteria under standard laboratory conditions. In conclusion, although NAD+-dependent DNA ligase is essential for mycobacterial viability, only low levels of protein are required for growth. These findings suggest that very efficient inhibition of enzyme activity would be required if NAD+-dependent DNA ligase is to be useful as an antibiotic target in mycobacteria. The strains developed here will provide useful tools for the evaluation of the efficacy of any appropriate compounds in mycobacteria

Open-canopy ponds benefit diurnal pollinator communities in an agricultural landscape: implications for farmland pond management

1. Declines in pollinating invertebrates across intensively cultivated landscapes linked to reductions in flower-rich habitats constitute a key threat to biodiversity conservation and the provision of ecosystem services. Over recent decades, many ponds in agricultural landscapes have become overgrown with woody vegetation, resulting in heavily shaded, flower-poor pond basins and margins. Restoration of farmland ponds through removal of sediment and encroaching woody vegetation (canopy management) from pond margins greatly enhances freshwater biodiversity. Nevertheless, the consequences of pond management for pond-margin plants and pollinating insects remain poorly understood. Here, we studied these effects for ponds in Norfolk, eastern England. 2. We compared richness, abundance and composition of pollinating insects (hymenopterans and syrphids) and insect-pollinated plant communities between open-canopy pond systems subjected to either (i) long-term regular management of woody vegetation or (ii) recent restoration by woody vegetation and sediment removal with those communities at (iii) ponds dominated by woody vegetation. 3. Canopy management increased the richness and abundance of pollinators and insect-pollinated plants. Pollinator richness and abundance was best explained by improvements in flower resources at open-canopy ponds. Management most strongly influenced hymenopteran communities. 4. Ponds represent important semi-natural habitats for insect-pollinated plant and pollinator communities in farmland. To enhance food resources, diversity and abundance of diurnal pollinators, conservation management at ponds should aim for mosaics of ponds at different successional stages with a high proportion of early successional open-canopy ponds. Agricultural ponds are emerging as important habitats not only for aquatic biodiversity, but also for terrestrial species, thus warranting their prioritisation in future agri-environment schemes

Factors affecting color correction of retroreflective markings

A nighttime field study was conducted to assess the effects of retroreflective material area, distribution, and color on judgments of conspicuity. Participants, seated in a stationary vehicle, took part in a pairwise comparison of the stimuli. The independent variables included retroreflective power, area and distribution of the retroreflective material, color of the retroreflective material, participant age, and participant gender. The results indicate that color (white, fluorescent yellow-green, and fluorescent red-orange) was a significant factor in the judgment of conspicuity, as might be predicted from the Helmholtz-Kohlrausch effect. In addition, color interacted with the distribution of material at the high level of retroreflective power. The area of the retroreflective material was also significant. The present study, in agreement with a number of previous studies, indicates that color influences the conspicuity of retroreflective stimuli, but that the results are not always in agreement with color correction factors prescribed in ASTM E 1501. The discrepancy between empirically derived color correction factors seems to be attributable to an interaction of the stimulus size (subtended angle) and color, which previous studies have not extensively examined. To a lesser degree, the retroreflective power of a material also appears to influence conspicuity. While the ASTM correction factors may be appropriate for intermediate subtended solid angles, particularly for nonsaturated colors, smaller correction factors appear appropriate for markings subtending small angles (approaching point sources), and larger factors for larger subtended angles of saturated stimuli.The University of Michigan Industry Affiliation Program for Human Factors in Transportation Safetyhttp://deepblue.lib.umich.edu/bitstream/2027.42/91263/1/102869.pd

The Effects of Lead-Vehicle Size on Driver Following Behavior: Is Ignorance Truly Bliss?

The objective of this study was to examine whether size of a lead vehicle (passenger car or light truck) affects the distance at which following vehicles travel. Naturalistic following data were collected from drivers using instrumented passenger cars in place of their own vehicles. The results show that these drivers followed light trucks at shorter distances than they followed other passenger cars by an average of 5.6 m, or .19 s in headway time margin, but at the same velocities and range-rates. This result is discussed in the context of a passenger car driver’s ability to see beyond a lead vehicle to assess, and respond to, the status of traffic downstream. The results of this study suggest that knowing the state of traffic beyond the lead vehicle, even by only one additional vehicle, affects gap length. Specifically, it appears that when dimensions of lead vehicles permit other drivers to see through, over, or around them, drivers maintain significantly longer (i.e., safer) distances